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goat anti mouse cd16 32  (R&D Systems)


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    R&D Systems goat anti mouse cd16 32
    Goat Anti Mouse Cd16 32, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+cd16+32/Mouse+Fc+gamma+RII%2FRIII+(CD32%2FCD16)+Antibody/pm40983457-66-26-30
    Average 94 stars, based on 33 article reviews
    goat anti mouse cd16 32 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: EDB-mediated neuroprotection against acute ischemic brain injury is associated with reduced central and peripheral inflammation
    Article Snippet: .. Then, the brain sections were blocked with 5% BSA in PBS for 1 h and stained with goat anti-CD16/32 (AF1460, R&D, Minneapolis, USA) and rabbit anti-Iba1 antibodies (Wako pure, Tokyo, Japan) overnight at 4°C. .. After 3 washes with PBST, the samples were treated with Alexa 647- and Alexa 488-conjugated secondary antibodies (Abcam, Cambridge, MA, USA) for 1 h. After another 3 washes, the slides were stained with DAPI and imaged on a confocal laser scanning microscope (Nikon C2si).

    Blocking Assay:

    Article Title: Role of IL-10 in Resolution of Inflammation and Functional Recovery after Peripheral Nerve Injury
    Article Snippet: .. To block nonspecific binding of antibodies, tissue sections were incubated in blocking solution (5% normal serum plus 2% ovalbumin in PBS with 0.3% Triton X-100) at room temperature for at least 1 h. Sections were then incubated overnight at 4°C with primary antibodies: goat anti-IL-10 (1:50; Santa Cruz Biotechnology, M-18, sc-1783), rat anti-CD11b monoclonal (1:200; AbD Serotec, MCA711); rabbit anti-Iba1 (1:400; Wako, 019-19741); rat anti-CD86 monoclonal (1:100; BD PharMingen, 550542), goat anti-CD206 (1:200; Santa Cruz Biotechnology, C-20, sc-34577); goat anti-CD16/32 (1:200; R&D Systems, AF1460), rabbit anti-CD163 (1:200; Santa Cruz Biotechnology, M-96, sc-33560); goat anti-arginase-1 (1:200; Santa Cruz Biotechnology, V-20, sc-18354); and rabbit anti-GAP-43 polyclonal (1:500; Millipore, AB5220). ..

    Article Title: Role of IL-10 in Resolution of Inflammation and Functional Recovery after Peripheral Nerve Injury
    Article Snippet: .. To block nonspecific binding of antibodies, tissue sections were incubated in blocking solution (5% normal serum plus 2% ovalbumin in PBS with 0.3% Triton X-100) at room temperature for at least 1 h. Sections were then incubated overnight at 4°C with primary antibodies: goat anti-IL-10 (1:50; Santa Cruz Biotechnology, M-18, sc-1783), rat anti-CD11b monoclonal (1:200; AbD Serotec, MCA711); rabbit anti-Iba1 (1:400; Wako, 019-19741); rat anti-CD86 monoclonal (1:100; BD PharMingen, 550542), goat anti-CD206 (1: 200; Santa Cruz Biotechnology, C-20, sc-34577); goat anti-CD16/32 (1:200; R&D Systems, AF1460), rabbit anti-CD163 (1:200; Santa Cruz Biotechnology, M-96, sc-33560); goat anti-arginase-1 (1:200; Santa Cruz Biotechnology, V-20, sc-18354); and rabbit anti-GAP-43 polyclonal (1:500; Millipore, AB5220). ..

    Binding Assay:

    Article Title: Role of IL-10 in Resolution of Inflammation and Functional Recovery after Peripheral Nerve Injury
    Article Snippet: .. To block nonspecific binding of antibodies, tissue sections were incubated in blocking solution (5% normal serum plus 2% ovalbumin in PBS with 0.3% Triton X-100) at room temperature for at least 1 h. Sections were then incubated overnight at 4°C with primary antibodies: goat anti-IL-10 (1:50; Santa Cruz Biotechnology, M-18, sc-1783), rat anti-CD11b monoclonal (1:200; AbD Serotec, MCA711); rabbit anti-Iba1 (1:400; Wako, 019-19741); rat anti-CD86 monoclonal (1:100; BD PharMingen, 550542), goat anti-CD206 (1:200; Santa Cruz Biotechnology, C-20, sc-34577); goat anti-CD16/32 (1:200; R&D Systems, AF1460), rabbit anti-CD163 (1:200; Santa Cruz Biotechnology, M-96, sc-33560); goat anti-arginase-1 (1:200; Santa Cruz Biotechnology, V-20, sc-18354); and rabbit anti-GAP-43 polyclonal (1:500; Millipore, AB5220). ..

    Article Title: Role of IL-10 in Resolution of Inflammation and Functional Recovery after Peripheral Nerve Injury
    Article Snippet: .. To block nonspecific binding of antibodies, tissue sections were incubated in blocking solution (5% normal serum plus 2% ovalbumin in PBS with 0.3% Triton X-100) at room temperature for at least 1 h. Sections were then incubated overnight at 4°C with primary antibodies: goat anti-IL-10 (1:50; Santa Cruz Biotechnology, M-18, sc-1783), rat anti-CD11b monoclonal (1:200; AbD Serotec, MCA711); rabbit anti-Iba1 (1:400; Wako, 019-19741); rat anti-CD86 monoclonal (1:100; BD PharMingen, 550542), goat anti-CD206 (1: 200; Santa Cruz Biotechnology, C-20, sc-34577); goat anti-CD16/32 (1:200; R&D Systems, AF1460), rabbit anti-CD163 (1:200; Santa Cruz Biotechnology, M-96, sc-33560); goat anti-arginase-1 (1:200; Santa Cruz Biotechnology, V-20, sc-18354); and rabbit anti-GAP-43 polyclonal (1:500; Millipore, AB5220). ..

    Incubation:

    Article Title: Role of IL-10 in Resolution of Inflammation and Functional Recovery after Peripheral Nerve Injury
    Article Snippet: .. To block nonspecific binding of antibodies, tissue sections were incubated in blocking solution (5% normal serum plus 2% ovalbumin in PBS with 0.3% Triton X-100) at room temperature for at least 1 h. Sections were then incubated overnight at 4°C with primary antibodies: goat anti-IL-10 (1:50; Santa Cruz Biotechnology, M-18, sc-1783), rat anti-CD11b monoclonal (1:200; AbD Serotec, MCA711); rabbit anti-Iba1 (1:400; Wako, 019-19741); rat anti-CD86 monoclonal (1:100; BD PharMingen, 550542), goat anti-CD206 (1:200; Santa Cruz Biotechnology, C-20, sc-34577); goat anti-CD16/32 (1:200; R&D Systems, AF1460), rabbit anti-CD163 (1:200; Santa Cruz Biotechnology, M-96, sc-33560); goat anti-arginase-1 (1:200; Santa Cruz Biotechnology, V-20, sc-18354); and rabbit anti-GAP-43 polyclonal (1:500; Millipore, AB5220). ..

    Article Title: Role of IL-10 in Resolution of Inflammation and Functional Recovery after Peripheral Nerve Injury
    Article Snippet: .. To block nonspecific binding of antibodies, tissue sections were incubated in blocking solution (5% normal serum plus 2% ovalbumin in PBS with 0.3% Triton X-100) at room temperature for at least 1 h. Sections were then incubated overnight at 4°C with primary antibodies: goat anti-IL-10 (1:50; Santa Cruz Biotechnology, M-18, sc-1783), rat anti-CD11b monoclonal (1:200; AbD Serotec, MCA711); rabbit anti-Iba1 (1:400; Wako, 019-19741); rat anti-CD86 monoclonal (1:100; BD PharMingen, 550542), goat anti-CD206 (1: 200; Santa Cruz Biotechnology, C-20, sc-34577); goat anti-CD16/32 (1:200; R&D Systems, AF1460), rabbit anti-CD163 (1:200; Santa Cruz Biotechnology, M-96, sc-33560); goat anti-arginase-1 (1:200; Santa Cruz Biotechnology, V-20, sc-18354); and rabbit anti-GAP-43 polyclonal (1:500; Millipore, AB5220). ..



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    Interchim Chemicals streptavidin fp647 interchim fp ca5640 goat anti hamster af488 thermo fisher scientific invitrogen a 21110 anti cd16 32
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    goat anti-cd16/32  (R&D Systems Hematology)
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    NKG2A+ NK cell line NK-92 cells were co-cultured with K562-HLA-E-VL9 target cells, at the indicated effector:target (E:T) ratios. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture. ( A ) Target cell killing of K562 or K562-HLA-E-VL9 target cells by NK-92. Dots represent means of independent experiments. ( B ) Target cell killing by NK-92 NK cells in the presence of increasing concentration of monoclonal antibodies: wildtype 3H4 IgG1 with low affinity of HLA-E-VL9 (left), affinity-matured 3H4 v31 IgG1 (middle), or anti-NKG2A (right). Dots and error bars represent mean and SEM of three-four replicates. (C) 3H4v31 mAb at 2 µg/mL was added to a culture of K562-HLA-E-VL9 target cells. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture. Dots represent means of independent experiments. (D) Target cell killing by <t>NK-92.CD16</t> with media versus 2 µg /mL of 3H4v31. Dots represent means of independent experiments. Statistical analysis was performed by two-sided paired t-tests. Asterisks indicate statistical significance between groups: * P<0.05, ** P<0.01.
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    goat anti cd16 32  (R&D Systems)
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    NKG2A+ NK cell line NK-92 cells were co-cultured with K562-HLA-E-VL9 target cells, at the indicated effector:target (E:T) ratios. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture. ( A ) Target cell killing of K562 or K562-HLA-E-VL9 target cells by NK-92. Dots represent means of independent experiments. ( B ) Target cell killing by NK-92 NK cells in the presence of increasing concentration of monoclonal antibodies: wildtype 3H4 IgG1 with low affinity of HLA-E-VL9 (left), affinity-matured 3H4 v31 IgG1 (middle), or anti-NKG2A (right). Dots and error bars represent mean and SEM of three-four replicates. (C) 3H4v31 mAb at 2 µg/mL was added to a culture of K562-HLA-E-VL9 target cells. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture. Dots represent means of independent experiments. (D) Target cell killing by <t>NK-92.CD16</t> with media versus 2 µg /mL of 3H4v31. Dots represent means of independent experiments. Statistical analysis was performed by two-sided paired t-tests. Asterisks indicate statistical significance between groups: * P<0.05, ** P<0.01.
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    goat anti cd16 32 antibody  (R&D Systems)
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    R&D Systems goat anti cd16 32 antibody
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    Image Search Results


    NKG2A+ NK cell line NK-92 cells were co-cultured with K562-HLA-E-VL9 target cells, at the indicated effector:target (E:T) ratios. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture. ( A ) Target cell killing of K562 or K562-HLA-E-VL9 target cells by NK-92. Dots represent means of independent experiments. ( B ) Target cell killing by NK-92 NK cells in the presence of increasing concentration of monoclonal antibodies: wildtype 3H4 IgG1 with low affinity of HLA-E-VL9 (left), affinity-matured 3H4 v31 IgG1 (middle), or anti-NKG2A (right). Dots and error bars represent mean and SEM of three-four replicates. (C) 3H4v31 mAb at 2 µg/mL was added to a culture of K562-HLA-E-VL9 target cells. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture. Dots represent means of independent experiments. (D) Target cell killing by NK-92.CD16 with media versus 2 µg /mL of 3H4v31. Dots represent means of independent experiments. Statistical analysis was performed by two-sided paired t-tests. Asterisks indicate statistical significance between groups: * P<0.05, ** P<0.01.

    Journal: bioRxiv

    Article Title: A high affinity monoclonal antibody against HLA-E-VL9 enhances natural killer cell anti-tumor killing

    doi: 10.1101/2024.07.08.602401

    Figure Lengend Snippet: NKG2A+ NK cell line NK-92 cells were co-cultured with K562-HLA-E-VL9 target cells, at the indicated effector:target (E:T) ratios. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture. ( A ) Target cell killing of K562 or K562-HLA-E-VL9 target cells by NK-92. Dots represent means of independent experiments. ( B ) Target cell killing by NK-92 NK cells in the presence of increasing concentration of monoclonal antibodies: wildtype 3H4 IgG1 with low affinity of HLA-E-VL9 (left), affinity-matured 3H4 v31 IgG1 (middle), or anti-NKG2A (right). Dots and error bars represent mean and SEM of three-four replicates. (C) 3H4v31 mAb at 2 µg/mL was added to a culture of K562-HLA-E-VL9 target cells. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture. Dots represent means of independent experiments. (D) Target cell killing by NK-92.CD16 with media versus 2 µg /mL of 3H4v31. Dots represent means of independent experiments. Statistical analysis was performed by two-sided paired t-tests. Asterisks indicate statistical significance between groups: * P<0.05, ** P<0.01.

    Article Snippet: Primary antibodies-Goat anti-CD56 (1/400, AF2408, R&D), Rabbit anti-cleaved Caspase-3 (1/400, 9661, Cell Signaling technologies), Goat anti-CD16/32 (1/50, AF1460 R&D).

    Techniques: Cell Culture, Lysis, Co-Culture Assay, Concentration Assay

    NK effectors were co-cultured with K562-HLA-E-VL9 target cells as the indicated effector:target (E:T) ratios. ADCC is assessed by degranulation of NK cells as measured by CD107a positivity with the addition of test antibodies to the co-culture. (A) Degranulation of NK-92 transfected with CD16 (NK-92.CD16) with addition of mAbs 3H4v31 IgG, no antibody (no Ab), isotype control (CH65), low affinity wildtype 3H4 (WT 3H4), 3H4v31 IgG with LALA Fc mutation (3H4v31_LALA), and anti-NKG2A. (B) Degranulation of human primary NK cells isolated from 5 individuals with addition of mAbs 3H4v31 IgG, no antibody (no Ab), isotype control (CH65), low affinity wildtype 3H4 (WT 3H4), 3H4v31 IgG with LALA Fc mutation (3H4v31_LALA), and anti-NKG2A. Statistical analysis was performed by two-sided paired t-tests. Asterisks indicate statistical significance between 3H4v31 and isotype control: * P<0.05, ** P<0.01, *** P<0.001

    Journal: bioRxiv

    Article Title: A high affinity monoclonal antibody against HLA-E-VL9 enhances natural killer cell anti-tumor killing

    doi: 10.1101/2024.07.08.602401

    Figure Lengend Snippet: NK effectors were co-cultured with K562-HLA-E-VL9 target cells as the indicated effector:target (E:T) ratios. ADCC is assessed by degranulation of NK cells as measured by CD107a positivity with the addition of test antibodies to the co-culture. (A) Degranulation of NK-92 transfected with CD16 (NK-92.CD16) with addition of mAbs 3H4v31 IgG, no antibody (no Ab), isotype control (CH65), low affinity wildtype 3H4 (WT 3H4), 3H4v31 IgG with LALA Fc mutation (3H4v31_LALA), and anti-NKG2A. (B) Degranulation of human primary NK cells isolated from 5 individuals with addition of mAbs 3H4v31 IgG, no antibody (no Ab), isotype control (CH65), low affinity wildtype 3H4 (WT 3H4), 3H4v31 IgG with LALA Fc mutation (3H4v31_LALA), and anti-NKG2A. Statistical analysis was performed by two-sided paired t-tests. Asterisks indicate statistical significance between 3H4v31 and isotype control: * P<0.05, ** P<0.01, *** P<0.001

    Article Snippet: Primary antibodies-Goat anti-CD56 (1/400, AF2408, R&D), Rabbit anti-cleaved Caspase-3 (1/400, 9661, Cell Signaling technologies), Goat anti-CD16/32 (1/50, AF1460 R&D).

    Techniques: Cell Culture, Co-Culture Assay, Transfection, Control, Mutagenesis, Isolation

    Flow cytometry analysis of 3H4v31 binding to SiHa (A), and SU86.86 (B). Left: Cells were stained with 4 µg/mL 3H4v31, then with an anti-human IgG AF647 secondary antibody (black unshaded peaks). An isotype control stain is shown for comparison (grey shaded peaks). Right: NK-92.CD16 cells were co-cultured with cell lines at E:T ratio of 2.5:1. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture Dots represent means of independent experiments. * P<0.05, *** P<0.001

    Journal: bioRxiv

    Article Title: A high affinity monoclonal antibody against HLA-E-VL9 enhances natural killer cell anti-tumor killing

    doi: 10.1101/2024.07.08.602401

    Figure Lengend Snippet: Flow cytometry analysis of 3H4v31 binding to SiHa (A), and SU86.86 (B). Left: Cells were stained with 4 µg/mL 3H4v31, then with an anti-human IgG AF647 secondary antibody (black unshaded peaks). An isotype control stain is shown for comparison (grey shaded peaks). Right: NK-92.CD16 cells were co-cultured with cell lines at E:T ratio of 2.5:1. Target cell lysis was quantified by 51 Cr release after 6 hours of effector and target co-culture Dots represent means of independent experiments. * P<0.05, *** P<0.001

    Article Snippet: Primary antibodies-Goat anti-CD56 (1/400, AF2408, R&D), Rabbit anti-cleaved Caspase-3 (1/400, 9661, Cell Signaling technologies), Goat anti-CD16/32 (1/50, AF1460 R&D).

    Techniques: Flow Cytometry, Binding Assay, Staining, Control, Comparison, Cell Culture, Lysis, Co-Culture Assay